Effect of some strains of Mycoplasma on serum and milk biochemistry of dairy cows

A s a result of repeated complain of dairy farms from mycoplasma mastitis, our study was performed to provide some more investigations about the charring role of Mycoplasma bovis (M. bovis) and Mycoplasma bovigenitalium (M. bovigenitalium) in this problem which may lead to good diagnosis and understand of the nature of the disease. Two hundred and sixty cows were examined out of a herd of 500 dairy cows from which 60 cows where clinically mastitic, M. bovis and M. bovigenitalium were mostly detected directly by culture method and indirectly by ELISA test. Biochemical analysis of serum and milk whey declared that mycoplasma induced mastitis is accompanied by significant increase of serum enzymatic activities of ALT,AST and ALP as will as urea concentration. The serum protein fractions of mastitic cows showed hypoalbuminemia and hyperglobulinemia (α2, β2, γ globulins and Total globulins) with reduced A/G ratio in addition changes in milk whey represented by elevation of milk whey proteins, serum albumin, immunoglobulin and other whey proteins beside significant elevation of sodium and chlorides, while decline of β lactoglobulin, a lactoalbumin, calcium and phosphorus was noticed. These changes may aid in detection of mastitic cases and help in rapid diagnosis of the disease.